Neuropeptide F inhibits dopamine neuron interference of long-term memory consolidation in Drosophila

Kuan-Lin Feng; Ju-Yun Weng; Chun-Chao Chen; Mohammed Bin Abubaker; Hsuan-Wen Lin; Ching-Che Charng; Chung-Chuan Lo; J. Steven de Belle; Tim Tully; Cheng-Chang Lien; Ann-Shyn Chiang

iScience (Dec 2021)

Neuropeptide F inhibits dopamine neuron interference of long-term memory consolidation in Drosophila

Kuan-Lin Feng,
Ju-Yun Weng,
Chun-Chao Chen,
Mohammed Bin Abubaker,
Hsuan-Wen Lin,
Ching-Che Charng,
Chung-Chuan Lo,
J. Steven de Belle,
Tim Tully,
Cheng-Chang Lien,
Ann-Shyn Chiang

Affiliations

Kuan-Lin Feng: Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan
Ju-Yun Weng: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan
Chun-Chao Chen: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan
Mohammed Bin Abubaker: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan
Hsuan-Wen Lin: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan
Ching-Che Charng: Institute of Systems Neuroscience and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Chung-Chuan Lo: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan; Institute of Systems Neuroscience and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
J. Steven de Belle: Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan; Department of Psychological Sciences, University of San Diego, San Diego, CA 92110, USA; School of Life Sciences, University of Nevada, Las Vegas, NV 89154, USA; MnemOdyssey LLC, Escondido, CA 92027, USA
Tim Tully: Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan; Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan
Cheng-Chang Lien: Institute of Neuroscience and Brain Research Center, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan; Corresponding author
Ann-Shyn Chiang: Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan; Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan; Institute of Systems Neuroscience and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan; Kaohsiung Medical University, Kaohsiung 80708, Taiwan; National Health Research Institutes, Zhunan 35053, Taiwan; China Medical University, Taichung 40402, Taiwan; Kavli Institute for Brain and Mind, University of California at San Diego, La Jolla, CA 92093-0526, USA; Corresponding author

Journal volume & issue: Vol. 24, no. 12
p. 103506

Abstract

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Summary: Long-term memory (LTM) formation requires consolidation processes to overcome interfering signals that erode memory formation. Olfactory memory in Drosophila involves convergent projection neuron (PN; odor) and dopaminergic neuron (DAN; reinforcement) input to the mushroom body (MB). How post-training DAN activity in the posterior lateral protocerebrum (PPL1) continues to regulate memory consolidation remains unknown. Here we address this question using targeted transgenes in behavior and electrophysiology experiments to show that (1) persistent post-training activity of PPL1-α2α′2 and PPL1-α3 DANs interferes with aversive LTM formation; (2) neuropeptide F (NPF) signaling blocks this interference in PPL1-α2α′2 and PPL1-α3 DANs after spaced training to enable LTM formation; and (3) training-induced NPF release and neurotransmission from two upstream dorsal-anterior-lateral (DAL2) neurons are required to form LTM. Thus, NPF signals from DAL2 neurons to specific PPL1 DANs disinhibit the memory circuit, ensuring that periodic events are remembered as consolidated LTM.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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