AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2

Acong Yang; Tie-Juan Shao; Xavier Bofill-De Ros; Chuanjiang Lian; Patricia Villanueva; Lisheng Dai; Shuo Gu

doi:10.1038/s41467-020-16533-w

Nature Communications (Jun 2020)

AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2

Acong Yang,
Tie-Juan Shao,
Xavier Bofill-De Ros,
Chuanjiang Lian,
Patricia Villanueva,
Lisheng Dai,
Shuo Gu

Affiliations

Acong Yang: RNA Mediated Gene Regulation Section; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute
Tie-Juan Shao: RNA Mediated Gene Regulation Section; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute
Xavier Bofill-De Ros: RNA Mediated Gene Regulation Section; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute
Chuanjiang Lian: RNA Mediated Gene Regulation Section; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute
Patricia Villanueva: RNA Mediated Gene Regulation Section; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute
Lisheng Dai: RNA Mediated Gene Regulation Section; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute
Shuo Gu: RNA Mediated Gene Regulation Section; RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute

DOI: https://doi.org/10.1038/s41467-020-16533-w
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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3′ end of microRNAs binds to the PAZ domain of Argonaute (AGO) proteins. Here the authors show that terminal nucleotidyltransferases TUT4/7 and exonuclease DIS3L2 induce tailing and decay of 3’ end exposed-microRNAs in AGO PAZ mutant expressing- or cancer cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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