Mouse Corneal Stroma Fibroblast Primary Cell Culture

Yujin Zhang; Yen-Chiao Wang; Okada Yuka; Lingling Zhang; Chia-Yang Liu

doi:10.21769/BioProtoc.1960

Bio-Protocol (Oct 2016)

Mouse Corneal Stroma Fibroblast Primary Cell Culture

Yujin Zhang,
Yen-Chiao Wang,
Okada Yuka,
Lingling Zhang,
Chia-Yang Liu

Affiliations

Yujin Zhang: School of Optometry, Indiana University, Bloomington, USA
Yen-Chiao Wang: School of Optometry, Indiana University, Bloomington, USA
Okada Yuka: School of Optometry, Indiana University, Bloomington, USADepartment of Ophthalmology, Wakayama Medical University, Wakayama, Japan
Lingling Zhang: School of Optometry, Indiana University, Bloomington, USA
Chia-Yang Liu: School of Optometry, Indiana University, Bloomington, USA

DOI: https://doi.org/10.21769/BioProtoc.1960
Journal volume & issue: Vol. 6, no. 19

Abstract

Read online

This protocol is developed for primary cell culture of cornea stromal keratocytes isolated from neonatal mouse eyeballs. It provides an optimal condition to isolate stromal keratocytes which maintain high viability for cell culture.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

About the journal