Phytochemical screening and anti-candida activities of Crocus cancellatus herb. Ethanol extract

Abstract

The antifungal, antioxidant activity, total tannin estimation, and phytochemical screening of Crocus Cancellatus extract were investigated. The best yield was obtained with ethanol solvent (36.1%). A significant amount of tannin was obtained in the plant (11.1%). In Antifungal testing against Candida spp. Results were obtained between 8.22 and 13.16 mm. The minimum and maximum results were obtained against Candida Krusei (8.22 mm) and Candida glabatra (13.16 mm) respectively. The minimum inhibitory concentration (MIC) value obtained against all candida strains resulted in the range of 12.5-25 μg/ml. The minimum inhibitory zone 0 mm of FLU/25 was obtained against Candida famata While the highest inhibitory zone of FLU/25 38.40 mm was obtained against Candida guilliermondii. In the antioxidant study, the maximum value (98.68%) was obtained at 0.2 mL, while the minimum value was obtained at 0.1 mL (96.60%). 10 compounds were determined in the content determination study with LC-MS/MS. Gallic acid (46965.14 µg/g), Quinic acid (1935.71 µg/g), and malic acid (3831.37µg/g) was obtained at the highest levels. Vanillic acid (138.2 µg/g), Protocatechuic acid (116.87µg/g), tr-Ferulic acid (51.17µg/g), Quercetin (20.56 µg/g), p-coumaric acid (12.8 µg/g), Chlorogenic acid (2.51 µg/g) and Salicylic acid (1.56 µg/g) were obtained quantitatively less. As a result of all these results, it was determined that the antioxidant activity of the plant is effective on pathogenic species thanks to its phenolic compounds.

Keywords

• crocus cancellatus
• anti-candida