Growth kinetics and morphological characteristics of the Vero cell line and primary heart cells treated with the plant hormones Auxin, Kinetin, and Gibberellin

Abstract

Plants produce various hormones that regulate their growth and development. In recent years, much attention has been paid to the effects of these hormones on animal cells. The present work aims to study the influence of three phytohormones: auxin, kinetin and gibberellin on the Vero cell line and primary cardiac cells. These two types of cells are used in the field of virology. The parameters studied are the viability, growth and morphology of the cells. The pH variation and glucose consumption in cell cultures. The results showed that gibberellin stimulates the growth and viability of Vero cells, while kinetin improves the growth of cardiac cells. Auxin also enhances the proliferation of both cell types; but its effect is less significant than that of other phytohormones. The increase in cell growth in the presence of phytohormones was indirectly confirmed by the consumption of glucose and the reduction of pH of the cell culture. This proliferative effect of plant hormones on Vero and Heart cells can be exploited in the field of vaccine production.

Keywords

• phytohormones
• auxin
• gibberellin
• kinetin
• vero cells
• primary cardiac cells
• cell growth