Assessment of Bacteriological Quality and Determination of Antibiogram of Bacteria Isolated from Water Used in Selected Hospitals within Port Harcourt City and Obio/Akpor Local Government Area of Rivers State

Abstract

Study’s Novelty/Excerpt • This study investigates the bacteriological quality and antibiogram profiles of water samples from hospitals in Port Harcourt Metropolis, highlighting significant contamination and multi-drug resistance among isolated bacterial species. • The research identifies the presence of various bacteria, including Staphylococcus sp., Bacillus sp., and Klebsiella sp., with notable resistance to commonly used antibiotics, underscoring the potential public health risks associated with hospital water sources. • This study's findings emphasize the need for rigorous water treatment protocols to ensure safe consumption and reduce the risk of antibiotic-resistant infections in hospital settings. Full Abstract Water is vital for life, and water void of bacterial contamination is vital for hospital consumption and use. This study aimed to determine the bacteriological quality and antibiogram of different water samples in some hospitals within Port Harcourt Metropolis. Water samples were collected in different hospitals in Port Harcourt and Obio/Akpor Local Government Area of Rivers State, Nigeria. Based on water dispensers, outdoor taps, washing hand faucets, and faucets in the toilet and theatre. The bacteriological quality of the water samples, coagulase, haemolysis, biofilm, starch, and antibiogram were determined using standard microbiological procedures. The mean range of the total heterotrophic bacterial, staphylococcal, faecal coliform, and total coliform counts of the water samples were 8.5±0.7×105 to 3.8±2.1×107, 1.2±0.2 to 2.8±0.3×105, 0.0±0.0 to 8.0±4.2×103 and 0.0±0.0 to 1.1±0.1×105 CFU/mL, respectively. The prevalence of the isolated bacteria is Staphylococcus sp (17.5%), Bacillus sp (12.5%), Enterobacter sp. (12.5%), Klebsiella sp. (10%), Citrobacter sp. (5%), Escherichia coli (2.5%) and Siccibacter sp. (2.5%). Staphylococcus, Bacillus, Enterobacter, Klebsiella, Citrobacter, Escherichia coli, and Siccibacter sp. were positive for haemolysis and α-amylase production, 80% of Staphylococcus sp were coagulase positive while 46, 40, 57 and 25% of Staphylococcus, Bacillus, Enterobacter, and Klebsiella sp produced biofilm. The antibiogram showed multi-drug resistance (0.2-1.0). Levofloxacin was 60% effective against Staphylococcus sp, while susceptibility of Klebsiella and Citrobacter sp to ofloxacin, gentamycin, nalidixic acid, and levofloxacin 66.7%. The water samples from these hospitals might not be good for drinking. Thus, treatment of water before use is recommended. The high antibiotic resistance could imply the emergence of resistant isolates in hospital water.

Keywords

• drinking water
• antibiogram
• hospital environment