Optimum Level of Lactobacillus plantarum Supplementation as Probiotic on In Vitro Digestibility and Rumen Fermentation Products in Thai Native Cattle

Abstract

The objective of this study was to determine in vitro gas production kinetics, and rumen fermentation products of total mixed ration (TMR) supplemented with different level of Lactobacillus plantarum supplementation in Thai native cattle. The experiment design was Completely Randomized Design (CRD). The in vitro procedure was completed using 200 mg DM of TMR and four different concentration of L. plantarum; CON (without supplementation of L. plantarum), T1 (107 CFU / mL of L. plantarum supplementation), T2 (108 CFU / mL of L. plantarum supplementation), and T3 (109 CFU / mL of L. plantarum supplementation). The samples were prepared in three replications and incubated for 96 hours for gas production. Rumen fermentation products (pH, NH3, VFA) and digestibility experiment were done in four replications and incubated for 24 hours. In vitro gas production for 96 hours, gas production from insoluble fraction (b), and potential extent of gas production (|a|+b) were improved in the T1 and T2 groups (P < 0.05). The in vitro dry matter and organic matter digestibility, and true digestibility were particularly enhanced in the T1 group compared to other treatments (P <0.05). The pH and NH3 in this study were significantly affected (P<0.05). The significant improvements on the in vitro rumen fermentation products and digestibility endorse L. plantarum supplementation at concentration of 107 CFU/mL as probiotic candidate.