Nature Communications (May 2019)

Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids

  • Christopher J. Vavricka,
  • Takanobu Yoshida,
  • Yuki Kuriya,
  • Shunsuke Takahashi,
  • Teppei Ogawa,
  • Fumie Ono,
  • Kazuko Agari,
  • Hiromasa Kiyota,
  • Jianyong Li,
  • Jun Ishii,
  • Kenji Tsuge,
  • Hiromichi Minami,
  • Michihiro Araki,
  • Tomohisa Hasunuma,
  • Akihiko Kondo

DOI
https://doi.org/10.1038/s41467-019-09610-2
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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Bioproduction of tetrahydropapaveroline (THP) is limited by the specificity of monoamine oxidase (MAO). Here, the authors identify an insect 3,4-dihydroxyphenylacetaldehyde synthase (DHPAAS) that can bypass MAO for direct aldehyde production and demonstrate bifunctional switching of DHPAAS for efficient THP production.