High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces

Batzorig Lombodorj; Horas Cendana Tseng; Hwan-You Chang; Yen-Wen Lu; Namnan Tumurpurev; Chun-Wei Lee; Batdemberel Ganbat; Ren-Guei Wu; Fan-Gang Tseng

doi:10.3390/mi11030275

Micromachines (Mar 2020)

High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces

Batzorig Lombodorj,
Horas Cendana Tseng,
Hwan-You Chang,
Yen-Wen Lu,
Namnan Tumurpurev,
Chun-Wei Lee,
Batdemberel Ganbat,
Ren-Guei Wu,
Fan-Gang Tseng

Affiliations

Batzorig Lombodorj: School of Information and Communication Technology, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia
Horas Cendana Tseng: Department of Engineering and System Science, Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu 30013, Taiwan
Hwan-You Chang: Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Yen-Wen Lu: Department of Biomechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan
Namnan Tumurpurev: Department of Mechanical Engineering, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia
Chun-Wei Lee: Department of Engineering and System Science, Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu 30013, Taiwan
Batdemberel Ganbat: Department of Physics, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia
Ren-Guei Wu: Department of Engineering and System Science, Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu 30013, Taiwan
Fan-Gang Tseng: Department of Engineering and System Science, Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu 30013, Taiwan

DOI: https://doi.org/10.3390/mi11030275
Journal volume & issue: Vol. 11, no. 3
p. 275

Abstract

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A microfluidic chip, which can separate and enrich leukocytes from whole blood, is proposed. The chip has 10 switchback curve channels, which are connected by straight channels. The straight channels are designed to permit the inertial migration effect and to concentrate the blood cells, while the curve channels allow the Dean flow to further classify the blood cells based on the cell sizes. Hydrodynamic suction is also utilized to remove smaller blood cells (e.g., red blood cell (RBC)) in the curve channels for higher separation purity. By employing the inertial migration, Dean flow force, and hydrodynamic suction in a continuous flow system, our chip successfully separates large white blood cells (WBCs) from the whole blood with the processing rates as high as 1 × 108 cells/sec at a high recovery rate at 93.2% and very few RBCs (~0.1%).

Published in Micromachines

ISSN: 2072-666X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Mechanical engineering and machinery
Website: https://www.mdpi.com/journal/micromachines

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