Revista Facultad Nacional de Agronomía Medellín (Jun 2008)
BEBIDA FERMENTADA DE SUERO DE QUESO FRESCO INOCULADA CON Lactobacillus casei FERMENTED FRESH CHEESE MILKWHEY BEVERAGE INOCULATED WITH Lactobacillus casei
Abstract
Este trabajo tuvo por objetivo desarrollar una bebida fermentada de suero de queso fresco inoculada con Lactobacillus casei, a la cual se le evaluó la viabilidad del microorganismo, utilizando medios de cultivo selectivos, bajo condiciones anaeróbicas y, luego, se procedió a verificar su resistencia a los ácidos gástricos y sales biliares, simulando así, las condiciones del tracto gastrointestinal de los humanos. Para verificar la supervivencia durante el período de almacenamiento (21 días), la cepa, se caracterizó morfológica y bioquímicamente. Adicionalmente, se llevó a cabo la prueba de aceptabilidad, evaluando la bebida con 80 jueces. Se realizaron análisis físico-químicos, microbiológicos y sensoriales, a la materia prima y al producto elaborado, acorde a las normas vigentes en Colombia. La bebida fue saborizada con pulpa de maracuyá (Passi flora edulis). Se obtuvieron recuentos de viabilidad a pH 2,0, en agar MRS de 5,38∙10(7)ufc∙g-1 y 1,3∙10(6)ufc∙g-1 y en agar M17 de 6,96∙10(7)ufc∙g-1 y de 1,16∙10(6)ufc∙g-1, en los días 1 y 21, respectivamente. A pH 7,0, en agar MRS, se registraron valores de 3,37 ∙10(7)ufc∙g-1 y 1.56 ∙10(6)ufc∙g-1 y en agar M17 de 8,85∙10(7)ufc∙g-1 y de 1,82∙10(6)ufc∙g-1, en los días 1 y 21, respectivamente. La bebida desarrollada, tuvo una aceptación de “me gusta”, y presentó una vida de anaquel de hasta 21 días.The objective of this research was to develop a fermented fresh cheese milkwhey beverage inoculated with Lactobacillus casei, to which the viability of microorganism was evaluated using selective cultivation means under anaerobic conditions and then it was verified its biliary resistance to gastric acids and salts, simulating this way, the conditions of gastrointestinal tract of humans. To verify the survival during the period of storage (21 days), the stump was characterized morphological and bio-chemically. Additionally the test of acceptability was carried out evaluating beverage with 80 judges. Physical-chemical, microbiological and sensorial analysis were made to raw material and to elaborated product, according to effective norms in Colombia . The beverage was flavored with pulp of maracuyá (Passi flora edulis). Counts of viability to pH 2.0 were obtained, in MRS agar of 5,38∙10(7)ufc∙g-1 and 1,3∙10(6)ufc∙g-1 and in M17 agar of 6,96∙17(7)ufc∙g-1 and 1,16∙10(6)ufc∙g- 1, in days 1 and 21, respectively. To pH 7,0, in MRS agar values of 3,37∙107ufc∙g-1 and 1,56∙10(6)ufc∙g-1 were obtained and in M17 agar, values of 8,85∙10(7)ufc∙g-1 and 1,82. ∙10(6)ufc∙g- 1, in days 1 and 21, respectively. The developed beverage had an acceptance of “I like”, and presented a shelf life up to 21 days.
