Differential proteomic patterns of plasma extracellular vesicles show potential to discriminate β-thalassemia subtypes

Na Li; Bowen Wu; Jifeng Wang; Yumeng Yan; Peng An; Yuezhen Li; Yuning Liu; Yanfei Hou; Xiaoqing Qing; Lili Niu; Xiang Ding; Zhensheng Xie; Mengmeng Zhang; Xiaojing Guo; Xiulan Chen; Tanxi Cai; Jianming Luo; Fudi Wang; Fuquan Yang

iScience (Feb 2023)

Differential proteomic patterns of plasma extracellular vesicles show potential to discriminate β-thalassemia subtypes

Na Li,
Bowen Wu,
Jifeng Wang,
Yumeng Yan,
Peng An,
Yuezhen Li,
Yuning Liu,
Yanfei Hou,
Xiaoqing Qing,
Lili Niu,
Xiang Ding,
Zhensheng Xie,
Mengmeng Zhang,
Xiaojing Guo,
Xiulan Chen,
Tanxi Cai,
Jianming Luo,
Fudi Wang,
Fuquan Yang

Affiliations

Na Li: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Bowen Wu: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Jifeng Wang: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Yumeng Yan: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Peng An: Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Yuezhen Li: Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Yuning Liu: Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Yanfei Hou: Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Xiaoqing Qing: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Lili Niu: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Xiang Ding: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Zhensheng Xie: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Mengmeng Zhang: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Xiaojing Guo: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Xiulan Chen: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Tanxi Cai: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China
Jianming Luo: Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021 China
Fudi Wang: The Fourth Affiliated Hospital, School of Public Health, State Key Laboratory of Experimental Hematology, Zhejiang University School of Medicine, Hangzhou 310058, China
Fuquan Yang: Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding author

Journal volume & issue: Vol. 26, no. 2
p. 106048

Abstract

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Summary: The observed specificity of β-thalassemia-subtype phenotypes makes new diagnostic strategies that complement current screening methods necessary to determine each subtype and facilitate therapeutic regimens for different patients. Here, we performed quantitative proteomics of plasma-derived extracellular vesicles (EVs) of β-thalassemia major (TM) patients, β-thalassemia intermedia (TI) patients, and healthy controls to explore subgroup characteristics and potential biomarkers. Plasma quantitative proteomics among the same cohorts were analyzed in parallel to compare the biomarker potential of both specimens. EV proteomics showed significantly more abnormalities in immunity and lipid metabolism in TI and TM, respectively. The differential proteomic patterns of EVs were consistent with but more striking than those of plasma. Notably, we also found EV proteins to have a superior performance for discriminating β-thalassemia subtypes. These findings allowed us to propose a diagnostic model consisting of five proteins in EVs with subtyping potential, demonstrating the ability of plasma-derived EVs for the diagnosis of β-thalassemia patients.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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