Frontiers in Plant Science (Nov 2023)
Tomato short internodes and pedicels encode an LRR receptor-like serine/threonine-protein kinase ERECTA regulating stem elongation through modulating gibberellin metabolism
- Xueya Zhao,
- Xueya Zhao,
- Xueya Zhao,
- Xueya Zhao,
- Xueya Zhao,
- Kunpeng Zhang,
- Kunpeng Zhang,
- Kunpeng Zhang,
- Kunpeng Zhang,
- Kunpeng Zhang,
- Huidong Zhang,
- Huidong Zhang,
- Huidong Zhang,
- Huidong Zhang,
- Huidong Zhang,
- Mengxi Bi,
- Mengxi Bi,
- Mengxi Bi,
- Mengxi Bi,
- Mengxi Bi,
- Yi He,
- Yi He,
- Yiqing Cui,
- Yiqing Cui,
- Changhua Tan,
- Changhua Tan,
- Changhua Tan,
- Changhua Tan,
- Changhua Tan,
- Jian Ma,
- Jian Ma,
- Jian Ma,
- Jian Ma,
- Jian Ma,
- Mingfang Qi,
- Mingfang Qi,
- Mingfang Qi,
- Mingfang Qi,
- Mingfang Qi
Affiliations
- Xueya Zhao
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Xueya Zhao
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Xueya Zhao
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Xueya Zhao
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural Affairs, Shenyang, China
- Xueya Zhao
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Kunpeng Zhang
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Kunpeng Zhang
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Kunpeng Zhang
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Kunpeng Zhang
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural Affairs, Shenyang, China
- Kunpeng Zhang
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Huidong Zhang
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Huidong Zhang
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Huidong Zhang
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Huidong Zhang
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural Affairs, Shenyang, China
- Huidong Zhang
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Mengxi Bi
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Mengxi Bi
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Mengxi Bi
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Mengxi Bi
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural Affairs, Shenyang, China
- Mengxi Bi
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Yi He
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Yi He
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Yiqing Cui
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Yiqing Cui
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Changhua Tan
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Changhua Tan
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Changhua Tan
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Changhua Tan
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural Affairs, Shenyang, China
- Changhua Tan
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Jian Ma
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Jian Ma
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Jian Ma
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Jian Ma
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural Affairs, Shenyang, China
- Jian Ma
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- Mingfang Qi
- College of Horticulture, Shenyang Agricultural University, Shenyang, China
- Mingfang Qi
- National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning), Shenyang, China
- Mingfang Qi
- Key Laboratory of Protected Horticulture (Shenyang Agricultural University), Ministry of Education, Shenyang, China
- Mingfang Qi
- Key Laboratory of Horticultural Equipment, Ministry of Agriculture and Rural Affairs, Shenyang, China
- Mingfang Qi
- Collaborative Innovation Center of Protected Vegetable Provincial Co-construction Surrounds Bohai Gulf Region, Shenyang, China
- DOI
- https://doi.org/10.3389/fpls.2023.1283489
- Journal volume & issue
-
Vol. 14
Abstract
Plant height is an important agronomic trait. Dwarf varieties present several advantages, such as lodging resistance, increased yield, and suitability for mechanized harvesting, which are crucial for crop improvement. However, limited research is available on dwarf tomato varieties suitable for production. In this study, we report a novel short internode mutant named “short internode and pedicel (sip)” in tomato, which exhibits marked internode and pedicel shortening due to suppressed cell elongation. This mutant plant has a compact plant structure and compact inflorescence, and has been demonstrated to produce more fruits, resulting in a higher harvest index. Genetic analysis revealed that this phenotype is controlled by a single recessive gene, SlSIP. BSA analysis and KASP genotyping indicated that ERECTA (ER) is the possible candidate gene for SlSIP, which encodes a leucine-rich receptor-like kinase. Additionally, we obtained an ER functional loss mutant using the CRISPR/Cas9 gene-editing technology. The 401st base A of ER is substituted with T in sip, resulting in a change in the 134th amino acid from asparagine (N) to isoleucine (I). Molecular dynamics(MD) simulations showed that this mutation site is located in the extracellular LRR domain and alters nearby ionic bonds, leading to a change in the spatial structure of this site. Transcriptome analysis indicated that the genes that were differentially expressed between sip and wild-type (WT) plants were enriched in the gibberellin metabolic pathway. We found that GA3 and GA4 decreased in the sip mutant, and exogenous GA3 restored the sip to the height of the WT plant. These findings reveal that SlSIP in tomatoes regulates stem elongation by regulating gibberellin metabolism. These results provide new insights into the mechanisms of tomato dwarfing and germplasm resources for breeding dwarfing tomatoes.
Keywords
