Isolation and primary identification of shiga toxin producing Escherichia coli O157 in dairy cattle

Abstract

During the last years, the significance of diseases associated etiologically to Shiga-toxin producing Escherichia coli (STEC) is continuously increasing at a global scale, while the O157 serotype is con-sidered as one of the most important pathogens of animal origin. Large ruminants play a key role in the epidemiology of E. coli diseases among men. Bovine faeces are a primary source of contamina-tion of the environment and foods with this agent. The purpose of this study was to test a specific, microbiological algorithm for primary identification of STEC isolates from bovine faeces using sorbi-tol McConkey agar supplemented with cefixime and tellurite. The attempts were focused not only on increasing the sensitivity and specificity of serotype identification, but also on optimisation of labour and analysis costs. From May 2013 to October 2014, a total number of 1104 faecal swab samples from calves 3 to 6 months of age were collected from 19 farms in different administrative and geo-graphical regions of Bulgaria. Thirty six sorbitol-negative E. coli isolates (3.26%) were detected as belonging to the O157 serotype after slide agglutination test.

Keywords

• cattle
• Escherichia coli O157
• faeces
• isolation