Breaking Buds—Stop and Go? Acid Invertase Activities in Apple Leaf Buds during Dormancy Release until Bud Break

Abstract

Bud dormancy enables deciduous fruit trees to endure unfavorable conditions during winter, and considerably impacts growth and reproduction in spring. We investigated acid invertase activities during dormancy release until bud break under natural (orchard) conditions in two consecutive years. Our aim was to relate the activity patterns to the developmental progression and to air temperature, which is a major factor influencing the developmental processes at that stage. The enzyme assays were performed on extracts from leaf buds of the cultivar Idared, sampled from early March to April in the years 2020 and 2021. The air temperature was continually monitored during the observation periods. cwINV activity showed a trend of slight increase at the earlier developmental stages and sharply increased during bud break in both years. cwINV is known to contribute to organ sink strength. Its up-regulation may, therefore, be related to the increasing developmental demand for carbohydrates in apple leaf buds during dormancy release until bud break. vacINV activity was relatively constant at the earlier stages and also showed a pronounced increase in activity during bud break in both years. However, in both years, we observed drops in vacINV activity following cold spells. vacINV activity has been associated with growth via cell elongation through the regulation of cell turgor and may, thus, be involved in bud break. Therefore, we suggest that the down-regulation of vacINV activity as a consequence of cold spells might contribute to a delay in bud break to protect young leaf tissues from exposure to cold stress conditions.

Keywords

• acid invertase
• vacuolar invertase
• cell wall-bound invertase
• air temperature
• bud dormancy
• dormancy release