Molecules (Apr 2007)

Identificationof Major Phenolic Compounds of Chinese Water Chestnut and their Antioxidant Activity

  • Yanli You,
  • Xuewu Duan,
  • Xiaoyi Wei,
  • Xinguo Su,
  • Mouming Zhao,
  • Jian Sun,
  • Neungnapa Ruenroengklin,
  • Yueming Jiang

DOI
https://doi.org/10.3390/12040842
Journal volume & issue
Vol. 12, no. 4
pp. 842 – 852

Abstract

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Chinese water chestnut (CWC) is one of the most popular foods among Asian people due to its special taste and medical function. Experiments were conducted to test the antioxidant activity and then determine the major phenolic compound components present in CWC. CWC phenolic extract strongly inhibited linoleic acid oxidation and exhibited a dose-dependent free-radical scavenging activity against α,α-diphenyl-β-picrylhydrazyl (DPPH) radicals, superoxide anions and hydroxyl radicals, which was superior to ascorbic acid and butylated hydroxytoluene (BHT), two commercial used antioxidants. Furthermore, the CWC extract was found to have a relatively higher reducing power, compared with BHT. The major phenolic compounds present in CWC tissues were extracted, purified and identified by high-performance liquid chromatograph (HPLC) as (–)-gallocatechin gallate, (–)-epicatechin gallate and (+)-catechin gallate. This study suggests that CWC tissues exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

Keywords