Application of fluorescence spectroscopy as a field method in the determination of varietal differences radish (Raphanus sativus) accessions after harvesting

Abstract

The study aims to establish the application of fluorescence spectroscopy as a field method for determining post-harvest varietal differences in radish accessions (Raphanus sativus). The proposed method includes studies of radish root crops from standard varieties and those from first-generation hybrids employing fluorescence spectra. The experimental studies were carried out locally on the farm. The spectral installation for generating emission fluorescence spectra is mobile. In its setup (optical setup), a system engineering approach based on the classical principles of modern optoelectronics was applied. The experiment results can be used to optimize the time for analyzing the varietal difference of radish genotypes after harvest under uncontrolled conditions. The stability of the breeding variety and its common blacks with an established variety of the same species can be observed by monitoring the intensity of the signal. This will support the process of determining the belonging of a specific variety to a given variety, even for samples of unknown origin, when it is necessary to qualify the results of the samples in a short time.

Keywords

• radish accessions (Raphanus sativus L.var. sativus)
• varietal differences
• uncontrolled conditions
• field method
• fluorescence spectroscopy