A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care

Deding Tang; Shuang Wu; Mengqi Kong; Zhaonan Liu; Zonghao Li; Ying Han; Yan Gong; Jie Hu

doi:10.3390/bios14080381

Biosensors (Aug 2024)

A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care

Deding Tang,
Shuang Wu,
Mengqi Kong,
Zhaonan Liu,
Zonghao Li,
Ying Han,
Yan Gong,
Jie Hu

Affiliations

Deding Tang: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Shuang Wu: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Mengqi Kong: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Zhaonan Liu: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Zonghao Li: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Ying Han: School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China
Yan Gong: School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China
Jie Hu: Suzhou Diyinan Biotech Company, Suzhou 215129, China

DOI: https://doi.org/10.3390/bios14080381
Journal volume & issue: Vol. 14, no. 8
p. 381

Abstract

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Exercise-induced muscle injury is one of the most common types of sports injuries. Skeletal muscle troponin I (skTnI) serves as an ideal biomarker in assessing such injuries, facilitating timely detection and evaluation. In this study, we develop a fluorescent sandwich lateral flow immunoassay (LFIA) combined with a desktop analyzer for rapid detection of skTnI. Through optimizing the reaction system, the assay achieves a satisfying detection performance, reaching a limit of detection (LOD) of 0.5 ng/mL with a turnaround time of 15 min. The proposed detection platform offers portability, ease of use, and high sensitivity, which facilitates the monitoring of exercise-induced muscle injuries at the point of care. This feature is particularly advantageous for end users, enabling timely detection of sports-related injuries and ultimately enhancing prognosis and sports life.

Published in Biosensors

ISSN: 2079-6374 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.mdpi.com/journal/biosensors

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