Metabolic labelling of the carbohydrate core in bacterial peptidoglycan and its applications

Hai Liang; Kristen E. DeMeester; Ching-Wen Hou; Michelle A. Parent; Jeffrey L. Caplan; Catherine L. Grimes

doi:10.1038/ncomms15015

Nature Communications (Apr 2017)

Metabolic labelling of the carbohydrate core in bacterial peptidoglycan and its applications

Hai Liang,
Kristen E. DeMeester,
Ching-Wen Hou,
Michelle A. Parent,
Jeffrey L. Caplan,
Catherine L. Grimes

Affiliations

Hai Liang: Department of Chemistry and Biochemistry, University of Delaware
Kristen E. DeMeester: Department of Chemistry and Biochemistry, University of Delaware
Ching-Wen Hou: Department of Chemistry and Biochemistry, University of Delaware
Michelle A. Parent: Department of Medical Laboratory Sciences, University of Delaware
Jeffrey L. Caplan: Department of Biological Sciences, University of Delaware
Catherine L. Grimes: Department of Chemistry and Biochemistry, University of Delaware

DOI: https://doi.org/10.1038/ncomms15015
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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N-acetyl-muramic acid (NAM) is a core component of the bacterial peptidoglycan (PG) cell wall, and is recognised by the innate immune system. Here the authors engineer Gram-negative and Gram-positive bacteria to incorporate a modified NAM into the backbone of PG, which can be labelled with click chemistry for imaging and tracking.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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