Effect of Using Different Compensation of Growth Regulators on Hypocotyls Callus Intiation and Differentiate of Medicago Sativa

Abstract

The effect of some growth regulators on intiation ,grwth and differtiate of Alfalfa callus was studied .The search results showed a distinction MS media supplemented with growth regulators such as Kin ( 0.1 mgl) +2,4-D( 1.0 mg l) were distinguished in promoting callus formation from leaves and roots of Medicago sativa plants at ratio 90 and 100% successively. On the other hand the medium Kin (2.0mgL) +2,4-D (2.0mgL) formed callus from hypocotyl stem of seedlings in addition to formation of regeneration branches at number reached to 24 and of ratio 20% in one period.

Keywords

• growth regulators
• medicago sativa
• callus intiation and differentiate