Nature Communications (Aug 2024)
Discovery of an embryonically derived bipotent population of endothelial-macrophage progenitor cells in postnatal aorta
- Anna E. Williamson,
- Sanuri Liyanage,
- Mohammadhossein Hassanshahi,
- Malathi S. I. Dona,
- Deborah Toledo-Flores,
- Dang X. A. Tran,
- Catherine Dimasi,
- Nisha Schwarz,
- Sanuja Fernando,
- Thalia Salagaras,
- Aaron Long,
- Jan Kazenwadel,
- Natasha L. Harvey,
- Grant R. Drummond,
- Antony Vinh,
- Vashe Chandrakanthan,
- Ashish Misra,
- Zoltan Neufeld,
- Joanne T. M. Tan,
- Luciano Martelotto,
- Jose M. Polo,
- Claudine S. Bonder,
- Alexander R. Pinto,
- Shiwani Sharma,
- Stephen J. Nicholls,
- Christina A. Bursill,
- Peter J. Psaltis
Affiliations
- Anna E. Williamson
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Sanuri Liyanage
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Mohammadhossein Hassanshahi
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Malathi S. I. Dona
- Cardiac Cellular Systems Laboratory, Baker Heart and Diabetes Institute
- Deborah Toledo-Flores
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Dang X. A. Tran
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Catherine Dimasi
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Nisha Schwarz
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Sanuja Fernando
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Thalia Salagaras
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Aaron Long
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Jan Kazenwadel
- Centre for Cancer Biology, University of South Australia and SA Pathology
- Natasha L. Harvey
- Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide
- Grant R. Drummond
- Department of Microbiology, Anatomy, Physiology and Pharmacology and Centre for Cardiovascular Biology and Disease Research, School of Agriculture, Biomedicine and Environment, La Trobe University
- Antony Vinh
- Department of Microbiology, Anatomy, Physiology and Pharmacology and Centre for Cardiovascular Biology and Disease Research, School of Agriculture, Biomedicine and Environment, La Trobe University
- Vashe Chandrakanthan
- Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide
- Ashish Misra
- Faculty of Medicine and Health, University of Sydney and Heart Research Institute
- Zoltan Neufeld
- School of Mathematics and Physics, The University of Queensland
- Joanne T. M. Tan
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Luciano Martelotto
- Adelaide Centre for Epigenetics and the South Australian Immunogenomics Cancer Institute, Faculty of Health and Medical Sciences, The University of Adelaide
- Jose M. Polo
- Adelaide Centre for Epigenetics and the South Australian Immunogenomics Cancer Institute, Faculty of Health and Medical Sciences, The University of Adelaide
- Claudine S. Bonder
- Centre for Cancer Biology, University of South Australia and SA Pathology
- Alexander R. Pinto
- Cardiac Cellular Systems Laboratory, Baker Heart and Diabetes Institute
- Shiwani Sharma
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Stephen J. Nicholls
- Monash Cardiovascular Research Centre, Monash University
- Christina A. Bursill
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- Peter J. Psaltis
- Vascular Research Centre, Heart and Vascular Program, Lifelong Health Theme, South Australian Health and Medical Research Institute
- DOI
- https://doi.org/10.1038/s41467-024-51637-7
- Journal volume & issue
-
Vol. 15,
no. 1
pp. 1 – 21
Abstract
Abstract Converging evidence indicates that extra-embryonic yolk sac is the source of both macrophages and endothelial cells in adult mouse tissues. Prevailing views are that these embryonically derived cells are maintained after birth by proliferative self-renewal in their differentiated states. Here we identify clonogenic endothelial-macrophage (EndoMac) progenitor cells in the adventitia of embryonic and postnatal mouse aorta, that are independent of Flt3-mediated bone marrow hematopoiesis and derive from an early embryonic CX3CR1+ and CSF1R+ source. These bipotent progenitors are proliferative and vasculogenic, contributing to adventitial neovascularization and formation of perfused blood vessels after transfer into ischemic tissue. We establish a regulatory role for angiotensin II, which enhances their clonogenic and differentiation properties and rapidly stimulates their proliferative expansion in vivo. Our findings demonstrate that embryonically derived EndoMac progenitors participate in local vasculogenic responses in the aortic wall by contributing to the expansion of endothelial cells and macrophages postnatally.
