Generation of a human iPSC line (MPIi008-A) from a patient with Denys-Drash syndrome

Markus C. Doeser; Julia Krygin; Albrecht Röpke; Dong Han; Roland Wedlich-Söldner; Hans R. Schöler; Hermann Pavenstädt; Kee-Pyo Kim

Stem Cell Research (Jul 2022)

Generation of a human iPSC line (MPIi008-A) from a patient with Denys-Drash syndrome

Markus C. Doeser,
Julia Krygin,
Albrecht Röpke,
Dong Han,
Roland Wedlich-Söldner,
Hans R. Schöler,
Hermann Pavenstädt,
Kee-Pyo Kim

Affiliations

Markus C. Doeser: Department of Nephrology and Rheumatology, Internal Medicine D, University Hospital Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany; Interdisciplinary Center for Clinical Research (IZKF), University of Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany
Julia Krygin: Department of Nephrology and Rheumatology, Internal Medicine D, University Hospital Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany
Albrecht Röpke: Institute of Human Genetics, University of Münster, Vesaliusweg 12-14, Münster 48149, Germany
Dong Han: Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany
Roland Wedlich-Söldner: Institute of Cell Dynamics and Imaging, University of Münster, Münster 48149, Germany; Medical Faculty, University of Münster, Domagkstrasse 3, Münster 48149, Germany
Hans R. Schöler: Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany; Medical Faculty, University of Münster, Domagkstrasse 3, Münster 48149, Germany
Hermann Pavenstädt: Department of Nephrology and Rheumatology, Internal Medicine D, University Hospital Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany; Medical Faculty, University of Münster, Domagkstrasse 3, Münster 48149, Germany
Kee-Pyo Kim: Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany; Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, Republic of Korea; Corresponding author.

Journal volume & issue: Vol. 62
p. 102826

Abstract

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An induced pluripotent stem cell (hiPSC) line (MPIi008-A) was generated from fibroblasts of a 1-year-old male patient with Denys-Drash syndrome using lentiviral delivery of reprogramming factors OCT4, SOX2, KLF4 and c-MYC. The MPIi008-A iPSC line exhibited typical iPSC morphology and normal karyotype, expressed pluripotent stem cell markers, and showed developmental potential to differentiate into derivatives of all three germ layers in vivo. The hiPSC line harbours a heterozygous missense mutation (R394L) in exon 9 of the WT1 gene.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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