Jichu yixue yu linchuang (Nov 2022)

miR-760 inhibits proliferation, invasion and migration of human esophageal squamous cell carcinoma cell lines

  • YUAN Sheng-wu, CHEN Juan, LIU Kai

DOI
https://doi.org/10.16352/j.issn.1001-6325.2022.11.1697
Journal volume & issue
Vol. 42, no. 11
pp. 1697 – 1703

Abstract

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Objective To investigate the effects of miR-760 on the proliferation, invasion and migration of esophageal squamous cell carcinoma (ESCC) cells through targeted regulation of basic leucine zipper ATF-like transcription factor 3 (BATF3). Methods The cancer tissues and adjacent tissues of ESCC patients were collected, and normal human esophageal squamous epithelial cells Het-1A and human ESCC cell lines EC9706, KYSE150, ECA109, TE-10 were cultured in vitro. RT-qPCR was used to detect the expression of miR-760 and BATF3 mRNA. miR-760-mimics were transfected into TE-10 cells for up-regulating the expression of miR-760. CCK-8 method was used to detect cell proliferation and Transwell chamber was used to detect cell invasion as well as migration. Western blot was used to detect the expression of proliferation-related proteins cyclin D1, p21 and epithelial-mesenchymal transition related proteins MMP2, vimentin, and E-cadherin. Dual luciferase reporter gene experiment was used to confirm the targeting relationship between miR-760 and BATF3. Results Compared with the adjacent tissues and Het-1A cell, the expression of miR-760 was significantly reduced in ESCC tissues and in cell lines, while the expression of BATF3 increased (P＜0.05). The TE-10 cells with the highest expression of miR-760 were selected for subsequent experiments. Over-expression of miR-760 reduced the activity of TE-10 cells and the counting numbers of invasion and migration cells(P＜0.05). The expression of cyclin D1 and vimentin proteins increased the expression of p21 and E-cadherin proteins (P＜0.05). The results showed that miR-760 negatively regulated the expression of BATF3 (P＜0.05). Up-regulation of BATF3 expression reversed the inhibitory effects by over-expression of miR-760 on the proliferation, invasion and migration of TE-10 cells(P＜0.05). Conclusions Over-expression of miR-760 may inhibit the proliferation, invasion and migration of ESCC cells, which may be related to the targeted inhibition of BATF3 expression.

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