Down-regulation of LINC-ROR, HOXA-AS2 and MEG3 in gastric cancer

Shahrad Soghala; Kiana Harsiny; Parto Momeni; Mahsa Hatami; Vahid Kholghi Oskooei; Bashdar Mahmud Hussen; Mohammad Taheri; Soudeh Ghafouri-Fard

Heliyon (Oct 2022)

Down-regulation of LINC-ROR, HOXA-AS2 and MEG3 in gastric cancer

Shahrad Soghala,
Kiana Harsiny,
Parto Momeni,
Mahsa Hatami,
Vahid Kholghi Oskooei,
Bashdar Mahmud Hussen,
Mohammad Taheri,
Soudeh Ghafouri-Fard

Affiliations

Shahrad Soghala: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Kiana Harsiny: Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Parto Momeni: Department of Cellular and Molecular Biology-Molecular Cellular Science, Faculty of Basic Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Mahsa Hatami: Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Vahid Kholghi Oskooei: Department of Medical Biotechnology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran; Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Bashdar Mahmud Hussen: Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Kurdistan Region, Erbil, Iraq; Center of Research and Strategic Studies, Lebanese French University, Kurdistan Region, Erbil, Iraq
Mohammad Taheri: Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Institute of Human Genetics, Jena University Hospital, Jena, Germany; Corresponding author.
Soudeh Ghafouri-Fard: Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author.

Journal volume & issue: Vol. 8, no. 10
p. e11155

Abstract

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Long non-coding RNAs (lncRNAs) have been identified as modulators of gastric carcinogenesis. Evaluation of expression amounts of these transcripts is a primary but essential step for recognition of the role of lncRNAs in the carcinogenesis. Therefore, we compared expressions of LINC-ROR, HOXA-AS2, MEG3 and HOTTIP lncRNAs in gastric cancer samples and nearby non-cancerous samples. Expression levels of LINC-ROR, HOXA-AS2 and MEG3 lncRNAs have been lower in gastric cancer samples compared with nearby non-cancerous samples (Expression ratios = 0.26, 0.37 and 0.36; P values = 0.021, 0.015 and 0.032, respectively). However, expression levels of HOTTIP were not significantly different between gastric cancer tissues and nearby tissues (P value = 0.43). HOTTIP expression was associated with tumor size (P value = 0.04). In addition, MEG3 expression was associated with site of primary tumor (P = 0.0003). Expressions of LINC-ROR and HOXA-AS2 were not associated with any clinical or pathological parameter. ROC curve analysis revealed that HOXA-AS2 and LINC-ROR could significantly differentiate between gastric cancer samples and nearby non-cancerous tissues (AUC values = 0.68 and 0.64; P values = 0.01 and 0.04, respectively). Taken together, the current investigation provides clues for contribution of LINC-ROR, HOXA-AS2 and MEG3 lncRNAs in gastric carcinogenesis and warrants further mechanistical assays.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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