Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

Dacheng Ma; Zhimeng Xu; Zhaoyu Zhang; Xi Chen; Xiangzhi Zeng; Yiyang Zhang; Tingyue Deng; Mengfei Ren; Zheng Sun; Rui Jiang; Zhen Xie

doi:10.1038/s41467-019-08395-8

Nature Communications (Feb 2019)

Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

Dacheng Ma,
Zhimeng Xu,
Zhaoyu Zhang,
Xi Chen,
Xiangzhi Zeng,
Yiyang Zhang,
Tingyue Deng,
Mengfei Ren,
Zheng Sun,
Rui Jiang,
Zhen Xie

Affiliations

Dacheng Ma: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Zhimeng Xu: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Zhaoyu Zhang: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Xi Chen: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Xiangzhi Zeng: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Yiyang Zhang: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Tingyue Deng: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Mengfei Ren: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Zheng Sun: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Rui Jiang: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University
Zhen Xie: MOE Key Laboratory of Bioinformatics and Bioinformatics Division, Center for Synthetic and System Biology, Department of Automation, Beijing National Research Center for Information Science and Technology, Tsinghua University

DOI: https://doi.org/10.1038/s41467-019-08395-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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