Indian Journal of Ophthalmology (Jan 2023)

Practical tips and common mistakes in ocular microbiology sampling and processing

  • Sanchita Mitra,
  • Kavya Chandran,
  • Merle Fernandes

DOI
https://doi.org/10.4103/ijo.IJO_2190_22
Journal volume & issue
Vol. 71, no. 5
pp. 1698 – 1705

Abstract

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Ocular microbiology deals with miniscule samples from ocular infections, which are difficult to collect, process, and analyze, necessitating special skills, and the knowledge of troubleshooting errors to reach a specific diagnosis. In this article, we highlight several practical points in ocular microbiology, common mistakes, and various approaches to resolve them. We have covered sample collection from different ocular compartments, processing for smear preparation and culture, transport of samples, staining and reagents issues, artifacts and contaminants, and interpretation of in-vitro antimicrobial susceptibility testing reports. This review aims to help both ophthalmologists and microbiologists in making the practice of ocular microbiology and the interpretation of reports more reliable, hassle-free, and precise.

Keywords