Network during light-induced cotyledons opening and greening in Astragalus membranaceus

Abstract

Opening and greening are main characteristics of morphogenesis of cotyledons. For revealing interrelationship between metabolism and morphogenesis, metabolic shifts were analyzed in cotyledon of A. membranaceus seedlings with different stages in light and in darkness. Light induced 69 metabolites (MA), related to cotyledon greening; additional 89 metabolites (MB), related to cotyledon opening, were identified by WGCNA. The screening of metabolites shared in both MA and MB obtained 37 specific metabolites (MC) related to both opening and greening. In this context, main changes in MC occurred during A3, the stage in which cotyledons fully opened and greened. Within MC, few sugars, including L-(-)-sorbose, mannose, glucose and its derivatives, markedly decreased, while other sugars, amino acids, and unsaturated fatty acids increased. Most isoflavones and flavonols including ononin, caycosin-7-glucosides, quercetin, genistein, and catechin increased 5.3, 5.5, 13.4, 6.4 and 1.8 times, respectively. Thus, accumulated flavonoids play an important role during this developmental stage.

Keywords

• morphogenesis of cotyledon
• primary metabolites
• phenols
• fatty acids