Italian Journal of Animal Science (Jan 2021)

L-Arginine inhibits hydrogen peroxide-induced oxidative damage and inflammatory response by regulating antioxidant capacity in ovine intestinal epithelial cells

  • Hao Zhang,
  • Ying Zhang,
  • Xiaoyun Liu,
  • Mabrouk Elsabagh,
  • Yin Yu,
  • Along Peng,
  • Sifa Dai,
  • Hongrong Wang

DOI
https://doi.org/10.1080/1828051X.2021.1973916
Journal volume & issue
Vol. 20, no. 1
pp. 1620 – 1632

Abstract

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Little is known how L-arginine (Arg) affects the ovine intestinal epithelial cells (IOECs) redox status induced by hydrogen peroxide (H2O2). This study aimed to examine the impact of Arg on IOECs subjected to H2O2-induced oxidative damage, intestinal barrier injury, and inflammatory response. The IOECs were incubated for 16 h then classified as four groups (n = 6/group) and cultured in corresponding media including (1) control (CON) group, in which IOECs were cultured in Arg-free Dulbecco’s modified Eagle’s F12 Ham medium (DMEM) containing 100 μM Arg; (2) Arg group, in which IOECs were cultured in Arg-free DMEM containing 350 μM Arg; (3) H2O2 group, in which IOECs were cultured in CON group plus 150 μM H2O2; (4) Arg + H2O2 group, in which IOECs were cultured in Arg group plus150 μM H2O2. After culturing for 24 h in media, some characteristics of cells in the four groups were measured. Arg administration decreased the H2O2-induced reactive oxygen species (ROS) production compared with the H2O2 group (p < .05). Compared with H2O2, adding Arg to H2O2 increased (p < .05) transepithelial electrical resistance (TEER), but decreased (p < .05) tumour necrosis factor α (TNF-α) within the IOECs. Compared with H2O2, adding Arg to H2O2-induced injured IOECs increased (p < .05) glutathione peroxidase 1 (GPx1), zonula occludens-1 (ZO-1), and epithelial nitric oxide (NO) synthase (eNOS) protein levels, and decreased (p < .05) TNF-α levels within cells. Arg inhibits H2O2-induced oxidative damage, intestinal barrier injury, and inflammatory response by NO pathway within IOECs.HIGHLIGHTS Arg inhibits H2O2-induced oxidative damage of IOECs. Arg inhibits H2O2-induced barrier injury of IOECs. Arg inhibits H2O2-induced inflammatory of IOECs. Arg modulates the antioxidant activity by a NO pathway in IOECs.

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