Bioautomation (Dec 2007)

Immobilization of Biocatalysts and Cells on Hybrid Membranes Syntheses on Sol-gel Method

  • Yotova L.,
  • Marinkova D.,
  • Yaneva S.,
  • Samuneva B.

Journal volume & issue
Vol. 8, no. 1
pp. 172 – 183

Abstract

Read online

The investigations in the area of enzyme action in the living organisms give us the opportunity for applications of these biochemical catalysts in the different purposes of medicine, industry and analytical practice. Using of soluble enzymes is connected with many difficulties, because the enzymes are no regenerative and they are instability. By immobilization of the enzymes on to different carriers the more of these problems are overcome. The application of soluble enzymes caused many difficulties, by the reason that the enzymes can not be regenerated. The immobilization of the enzymes by means of different reagents and carriers overcomes this problem. The aim of this study to develop a sol-gel method of synthesis of new hybrid membrane, with immobilized biocatalysts (microbial cells and enzymes) for biosensor construction. This study shows that hybrid organic-inorganic membranes were synthesized.

Keywords