Nature Communications (Mar 2021)
Synchronous RNA conformational changes trigger ordered phase transitions in crystals
- Saminathan Ramakrishnan,
- Jason R. Stagno,
- Chelsie E. Conrad,
- Jienyu Ding,
- Ping Yu,
- Yuba R. Bhandari,
- Yun-Tzai Lee,
- Gary Pauly,
- Oleksandr Yefanov,
- Max O. Wiedorn,
- Juraj Knoska,
- Dominik Oberthür,
- Thomas A. White,
- Anton Barty,
- Valerio Mariani,
- Chufeng Li,
- Wolfgang Brehm,
- William F. Heinz,
- Valentin Magidson,
- Stephen Lockett,
- Mark S. Hunter,
- Sébastien Boutet,
- Nadia A. Zatsepin,
- Xiaobing Zuo,
- Thomas D. Grant,
- Suraj Pandey,
- Marius Schmidt,
- John C. H. Spence,
- Henry N. Chapman,
- Yun-Xing Wang
Affiliations
- Saminathan Ramakrishnan
- Structural Biophysics Laboratory, National Cancer Institute
- Jason R. Stagno
- Structural Biophysics Laboratory, National Cancer Institute
- Chelsie E. Conrad
- Structural Biophysics Laboratory, National Cancer Institute
- Jienyu Ding
- Structural Biophysics Laboratory, National Cancer Institute
- Ping Yu
- Structural Biophysics Laboratory, National Cancer Institute
- Yuba R. Bhandari
- Structural Biophysics Laboratory, National Cancer Institute
- Yun-Tzai Lee
- Structural Biophysics Laboratory, National Cancer Institute
- Gary Pauly
- Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute
- Oleksandr Yefanov
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Max O. Wiedorn
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Juraj Knoska
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Dominik Oberthür
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Thomas A. White
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Anton Barty
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Valerio Mariani
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Chufeng Li
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Wolfgang Brehm
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- William F. Heinz
- Optical Microscopy and Analysis Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research
- Valentin Magidson
- Optical Microscopy and Analysis Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research
- Stephen Lockett
- Optical Microscopy and Analysis Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research
- Mark S. Hunter
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Sébastien Boutet
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Nadia A. Zatsepin
- Department of Physics, Arizona State University
- Xiaobing Zuo
- X-ray Science Division, Argonne National Laboratory
- Thomas D. Grant
- Department of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, SUNY University at Buffalo
- Suraj Pandey
- Kenwood Interdisciplinary Research Complex Physics Department, University of Wisconsin-Milwaukee
- Marius Schmidt
- Kenwood Interdisciplinary Research Complex Physics Department, University of Wisconsin-Milwaukee
- John C. H. Spence
- Department of Physics, Arizona State University
- Henry N. Chapman
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Yun-Xing Wang
- Structural Biophysics Laboratory, National Cancer Institute
- DOI
- https://doi.org/10.1038/s41467-021-21838-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 10
Abstract
Time-resolved crystallography (TRX) is used for monitoring only small conformational changes of biomacromolecules within the same lattice. Here, the authors report the interplay between synchronous molecular rearrangements and lattice phase transitions in RNA crystals, providing the basis for the investigation of large conformational changes using TRX.
