Nature Communications (Oct 2022)
Cell-specific regulation of gene expression using splicing-dependent frameshifting
- Jonathan P. Ling,
- Alexei M. Bygrave,
- Clayton P. Santiago,
- Rogger P. Carmen-Orozco,
- Vickie T. Trinh,
- Minzhong Yu,
- Yini Li,
- Ying Liu,
- Kyra D. Bowden,
- Leighton H. Duncan,
- Jeong Han,
- Kamil Taneja,
- Rochinelle Dongmo,
- Travis A. Babola,
- Patrick Parker,
- Lizhi Jiang,
- Patrick J. Leavey,
- Jennifer J. Smith,
- Rachel Vistein,
- Megan Y. Gimmen,
- Benjamin Dubner,
- Eric Helmenstine,
- Patric Teodorescu,
- Theodoros Karantanos,
- Gabriel Ghiaur,
- Patrick O. Kanold,
- Dwight Bergles,
- Ben Langmead,
- Shuying Sun,
- Kristina J. Nielsen,
- Neal Peachey,
- Mandeep S. Singh,
- W. Brian Dalton,
- Fatemeh Rajaii,
- Richard L. Huganir,
- Seth Blackshaw
Affiliations
- Jonathan P. Ling
- Departments of Pathology, Johns Hopkins University School of Medicine
- Alexei M. Bygrave
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Clayton P. Santiago
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Rogger P. Carmen-Orozco
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Vickie T. Trinh
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Minzhong Yu
- Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation
- Yini Li
- Departments of Pathology, Johns Hopkins University School of Medicine
- Ying Liu
- Wilmer Eye Institute, Johns Hopkins University School of Medicine
- Kyra D. Bowden
- Department of Biomedical Engineering, Johns Hopkins University
- Leighton H. Duncan
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Jeong Han
- Wilmer Eye Institute, Johns Hopkins University School of Medicine
- Kamil Taneja
- Wilmer Eye Institute, Johns Hopkins University School of Medicine
- Rochinelle Dongmo
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Travis A. Babola
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Patrick Parker
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Lizhi Jiang
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Patrick J. Leavey
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Jennifer J. Smith
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Rachel Vistein
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Megan Y. Gimmen
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Benjamin Dubner
- Department of Oncology, Johns Hopkins University School of Medicine
- Eric Helmenstine
- Department of Oncology, Johns Hopkins University School of Medicine
- Patric Teodorescu
- Department of Oncology, Johns Hopkins University School of Medicine
- Theodoros Karantanos
- Department of Oncology, Johns Hopkins University School of Medicine
- Gabriel Ghiaur
- Department of Oncology, Johns Hopkins University School of Medicine
- Patrick O. Kanold
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Dwight Bergles
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Ben Langmead
- Kavli Neuroscience Discovery Institute, Johns Hopkins University
- Shuying Sun
- Departments of Pathology, Johns Hopkins University School of Medicine
- Kristina J. Nielsen
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Neal Peachey
- Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation
- Mandeep S. Singh
- Wilmer Eye Institute, Johns Hopkins University School of Medicine
- W. Brian Dalton
- Department of Oncology, Johns Hopkins University School of Medicine
- Fatemeh Rajaii
- Wilmer Eye Institute, Johns Hopkins University School of Medicine
- Richard L. Huganir
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Seth Blackshaw
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-022-33523-2
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 14
Abstract
Precise and reliable gene delivery remains technically challenging. Here, the authors show that rationally designed frameshifting splicing can be used to express genes only in targeted cell types, with the potential to enhance the specificity AAV gene delivery.
