PLoS ONE (Jan 2006)

Electrochemically generated acid and its containment to 100 micron reaction areas for the production of DNA microarrays.

  • Karl Maurer,
  • John Cooper,
  • Marcelo Caraballo,
  • James Crye,
  • Dominic Suciu,
  • Andrey Ghindilis,
  • Joseph A Leonetti,
  • Wei Wang,
  • Francis M Rossi,
  • Axel G Stöver,
  • Christopher Larson,
  • Hetian Gao,
  • Kilian Dill,
  • Andy McShea

DOI
https://doi.org/10.1371/journal.pone.0000034
Journal volume & issue
Vol. 1
p. e34

Abstract

Read online

An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.