Engineered kinesin motor proteins amenable to small-molecule inhibition

Martin F. Engelke; Michael Winding; Yang Yue; Shankar Shastry; Federico Teloni; Sanjay Reddy; T. Lynne Blasius; Pushpanjali Soppina; William O. Hancock; Vladimir I. Gelfand; Kristen J. Verhey

doi:10.1038/ncomms11159

Nature Communications (Apr 2016)

Engineered kinesin motor proteins amenable to small-molecule inhibition

Martin F. Engelke,
Michael Winding,
Yang Yue,
Shankar Shastry,
Federico Teloni,
Sanjay Reddy,
T. Lynne Blasius,
Pushpanjali Soppina,
William O. Hancock,
Vladimir I. Gelfand,
Kristen J. Verhey

Affiliations

Martin F. Engelke: Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place
Michael Winding: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University
Yang Yue: Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place
Shankar Shastry: Department of Biomedical Engineering, Penn State University
Federico Teloni: Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place
Sanjay Reddy: Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place
T. Lynne Blasius: Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place
Pushpanjali Soppina: Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place
William O. Hancock: Department of Biomedical Engineering, Penn State University
Vladimir I. Gelfand: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University
Kristen J. Verhey: Department of Cell and Developmental Biology, University of Michigan, 109 Zina Pitcher Place

DOI: https://doi.org/10.1038/ncomms11159
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

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The use of specific small molecule inhibitors can contribute to the study of kinesins' cellular functions. Here the authors develop a chemical-genetic approach to engineer kinesin motors that can be efficiently inhibited upon addition of cell-permeable molecules.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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