Nature Communications (Feb 2023)

A versatile, high-efficiency platform for CRISPR-based gene activation

  • Amy J. Heidersbach,
  • Kristel M. Dorighi,
  • Javier A. Gomez,
  • Ashley M. Jacobi,
  • Benjamin Haley

DOI
https://doi.org/10.1038/s41467-023-36452-w
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 10

Abstract

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The generation of CRISPR-mediated transcriptional activation (CRISPRa)-competent cell lines pose significant technical challenges. Here the authors report a platform for production of CRISPRa-ready cell populations which they combine with optimised expressed and synthetic gRNA scaffolds to enhance functionality.