RLI2 regulates Arabidopsis female gametophyte and embryo development by facilitating the assembly of the translational machinery

Shi-Xia Yu; Li-Qin Hu; Lu-Han Yang; Tao Zhang; Ruo-Bing Dai; Yan-Jie Zhang; Zhi-Ping Xie; Wen-Hui Lin

Cell Reports (Jul 2023)

RLI2 regulates Arabidopsis female gametophyte and embryo development by facilitating the assembly of the translational machinery

Shi-Xia Yu,
Li-Qin Hu,
Lu-Han Yang,
Tao Zhang,
Ruo-Bing Dai,
Yan-Jie Zhang,
Zhi-Ping Xie,
Wen-Hui Lin

Affiliations

Shi-Xia Yu: The Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Collaborative Innovation Center of Agri-Seeds/Joint Center for Single Cell Biology, Shanghai Jiao Tong University, Shanghai 200240, China; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
Li-Qin Hu: The Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Collaborative Innovation Center of Agri-Seeds/Joint Center for Single Cell Biology, Shanghai Jiao Tong University, Shanghai 200240, China; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
Lu-Han Yang: The Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Tao Zhang: The Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Ruo-Bing Dai: Zhiyuan College, Shanghai Jiao Tong University, Shanghai 200240, China
Yan-Jie Zhang: The Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Zhi-Ping Xie: The Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
Wen-Hui Lin: The Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Collaborative Innovation Center of Agri-Seeds/Joint Center for Single Cell Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding author

Journal volume & issue: Vol. 42, no. 7
p. 112741

Abstract

Read online

Summary: Eukaryotic protein translation is a complex process that requires the participation of different proteins. Defects in the translational machinery often result in embryonic lethality or severe growth defects. Here, we report that RNase L inhibitor 2/ATP-BINDING CASSETTE E2 (RLI2/ABCE2) regulates translation in Arabidopsis thaliana. Null mutation of rli2 is gametophytic and embryonic lethal, whereas knockdown of RLI2 causes pleiotropic developmental defects. RLI2 interacts with several translation-related factors. Knockdown of RLI2 affects the translational efficiency of a subset of proteins involved in translation regulation and embryo development, indicating that RLI2 has critical roles in these processes. In particular, RLI2 knockdown mutant exhibits decreased expression of genes involved in auxin signaling and female gametophyte and embryo development. Therefore, our results reveal that RLI2 facilitates assembly of the translational machinery and indirectly modulates auxin signaling to regulate plant growth and development.

CP: Plants

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

About the journal

Abstract

Keywords