Морфологія (Mar 2018)
Morphometric characteristics of the resistant link of salivary gland’s hemomicrocircular rate of rats in chronic ethanol intoxication
Abstract
Abstract. Background. To date, numerous clinical observations and experimental data have proven that there is practically no such organ that would not be subjected to toxic effects of alcohol. Purpose of the work was to determine the dynamics of changes in the metric indices of the submandibular glands’ resistive link of the haemomicrovascular bed of the rats in chronic ethanol intoxication. Methods. The work was performed on 45 white, non-breeding rats. 5 animals were an intact group, which was washed out 4 times a day with isotonic sodium chloride solution, and 40 - experimental, which was injected intrastomach 1 times a day with 12 mg / kg 400 ethanol (in terms of pure alcohol). Animals were withdrawn from the experiment at 5, 9, 12 and 30 days by overdose of thiopental anesthesia (25 mg / kg). Sections of the submandibular glands were enclosed in epon-812 according to the generally accepted method. The mean values of the outer diameter and diameter of the arterioles lumen were determined using a microscope with a digital microphocamera Biorex 3 with an adapted for data research program. Results. In the morphometric study it was found that on the fifth day after ethanol introduction, the mean values of the outer diameter of the arterioles decreased significantly by 15.8%, compared with the control group, and amounted to 15.28 ± 0.06 μm. Also, the diameter of the arteriol lumen was reduced by 53.5%, compared with the control group (p<0.05), and equaled 7.47 ± 0.03 μm. The average thickness of the vascular wall significantly increased by 41.8% and was 3.91 μm. On the ninth day after the introduction of ethanol, the mean values of the outer diameter of the arterioles increased significantly by 27.4% compared to the fifth day of the experiment and amounted to 19.46 ± 0.04 μm, but slightly higher than the control group (p<0.05) by 7.3%. Relatively on the thirtieth day of the experimental model of chronic ethanol intoxication, the outer diameter of the arteriol wall was 18.39 ± 0.04 μm and increased by 0.8%, compared to the twelfth observation day, and by 1.4% compared with the control group of rats. Conclusion. In the early stages of observation, vasospasm is determined, which is confirmed by an increase in the thickness of the vascular wall by 41.8%. At the ninth day there is dilatation, which manifests itself in a significant increase in the diameter of the outer and the lumen, and the decrease in the thickness of the vascular wall. The normalization of the indicators by the thirtieth day is not determined.
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