Extraction of globulin and albumin from Cerasus humilis by binary aqueous two-phase system

Abstract

In order to optimize the extraction conditions, an binary aqueous two-phase system (BATPS) composed of ethanol and acetone(volume ratio is 1∶2) and ammonium sulphate is selected to extract globulin and albumin from Cerasus humilis seeds. The distribution characteristic of the globulin and albumin in this aqueous two-phase system is reflected by comparing critical data such as partition coefficient and recovery rate. The experiment shows that the optimum conditions of albumin is composed of 27.5% ethanol and acetone (volume ratio is 1∶2), and 26% ammonium sulphate, the optimum conditions of globulin is composed of 17.5% ethanol and acetone(volume ratio is 1∶2), and 24% ammonium sulphate. These results suggest that the BATPS is efficient in extracting albumin and globulin with bright application prospect, and has great potential to be used in natural protein extraction.

Keywords

• other disciplines of bioengineering
• binary aqueous two-phase system (BATPS)
• extraction
• globulin
• albumin