Journal of Inflammation Research (Dec 2020)
Evaluation of Analgesic and Anti-inflammatory Potential of 80% Methanol Leaf Extract of Otostegia integrifolia Benth (Lamiaceae)
Abstract
Rediet Tesfaye,1 Abel Degu,1 Besufekad Abebe,2 Hiwot Ayalew2 1Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, EthiopiaCorrespondence: Abel DeguDepartment of Pharmacology and Clinical Pharmacy School of Pharmacy, College of Health Sciences, Addis Ababa University, PO Box 1176, Addis Ababa, EthiopiaEmail [email protected]: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice.Methods: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control.Results: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P< 0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P< 0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P< 0.05 and P< 0.001, respectively).Conclusion: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice.Keywords: analgesic activity, anti-inflammatory, hot plate, Otostegia integrifolia, carrageenan
