Protocol for the longitudinal study of neuroinflammation and reactive astrocytes in Lcn2CreERT2 mice

Teresa Ubina; William Agnew-Svoboda; Zoe A. Figueroa; Emma H. Wilson; Todd A. Fiacco; Martin M. Riccomagno

STAR Protocols (Dec 2024)

Protocol for the longitudinal study of neuroinflammation and reactive astrocytes in Lcn2CreERT2 mice

Teresa Ubina,
William Agnew-Svoboda,
Zoe A. Figueroa,
Emma H. Wilson,
Todd A. Fiacco,
Martin M. Riccomagno

Affiliations

Teresa Ubina: Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Department of Molecular, Cell, and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA
William Agnew-Svoboda: Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Department of Molecular, Cell, and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA
Zoe A. Figueroa: Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521, USA
Emma H. Wilson: Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Biomedical Sciences Graduate Program, University of California, Riverside, Riverside, CA 92521, USA; Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521, USA
Todd A. Fiacco: Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Biomedical Sciences Graduate Program, University of California, Riverside, Riverside, CA 92521, USA; Department of Molecular, Cell, and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA; Corresponding author
Martin M. Riccomagno: Neuroscience Graduate Program, Univeristy of California, Riverside, Riverside, CA 92521, USA; Biomedical Sciences Graduate Program, University of California, Riverside, Riverside, CA 92521, USA; Department of Molecular, Cell, and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA; Corresponding author

Journal volume & issue: Vol. 5, no. 4
p. 103322

Abstract

Read online

Summary: During brain disease, astrocytes can reprogram into a reactive state that alters many of their functions. Here, we present a protocol for studying neuroinflammation and reactive astrogliosis in mice using lipopolysaccharide (LPS) from E. coli. We describe steps for employing the Lcn2CreERT2 mouse crossed into a fluorescent Cre reporter line to label a subset of reactive astrocytes during and after inflammation. We then detail procedures for the longitudinal study of reactive astrocytes during the induction, progression, and/or resolution of astrogliosis.For complete details on the use and execution of this protocol, please refer to Agnew-Svoboda et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords