A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

Hongqing Chen; Fei Fei; Xinda Li; Zhenguo Nie; Dezhi Zhou; Libiao Liu; Jing Zhang; Haitao Zhang; Zhou Fei; Tao Xu

Bioactive Materials (Oct 2021)

A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

Hongqing Chen,
Fei Fei,
Xinda Li,
Zhenguo Nie,
Dezhi Zhou,
Libiao Liu,
Jing Zhang,
Haitao Zhang,
Zhou Fei,
Tao Xu

Affiliations

Hongqing Chen: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China; Department of Neurosurgery, Central Theater General Hospital, Wuhan, 430010, People's Republic of China
Fei Fei: Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China
Xinda Li: Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China; Key Laboratory for Advanced Materials Processing Technology, Ministry of Education, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China; Department of Neurosurgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China; Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, 610072, China
Zhenguo Nie: Department of Orthopedics, Fourth Medical Center of PLA General Hospital, 100048, Beijing, People's Republic of China
Dezhi Zhou: Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China; Key Laboratory for Advanced Materials Processing Technology, Ministry of Education, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China
Libiao Liu: Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China; Key Laboratory for Advanced Materials Processing Technology, Ministry of Education, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China
Jing Zhang: East China Institute of Digital Medical Engineering, Shangrao, 334000, People's Republic of China
Haitao Zhang: East China Institute of Digital Medical Engineering, Shangrao, 334000, People's Republic of China
Zhou Fei: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China; Corresponding author. Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China.
Tao Xu: Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China; Key Laboratory for Advanced Materials Processing Technology, Ministry of Education, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China; Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, 518055, People's Republic of China; Corresponding author. Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China.

Journal volume & issue: Vol. 6, no. 10
pp. 3580 – 3595

Abstract

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The ready-to-use, structure-supporting hydrogel bioink can shorten the time for ink preparation, ensure cell dispersion, and maintain the preset shape/microstructure without additional assistance during printing. Meanwhile, ink with high permeability might facilitate uniform cell growth in biological constructs, which is beneficial to homogeneous tissue repair. Unfortunately, current bioinks are hard to meet these requirements simultaneously in a simple way. Here, based on the fast dynamic crosslinking of aldehyde hyaluronic acid (AHA)/N-carboxymethyl chitosan (CMC) and the slow stable crosslinking of gelatin (GEL)/4-arm poly(ethylene glycol) succinimidyl glutarate (PEG-SG), we present a time-sharing structure-supporting (TSHSP) hydrogel bioink with high permeability, containing 1% AHA, 0.75% CMC, 1% GEL and 0.5% PEG-SG. The TSHSP hydrogel can facilitate printing with proper viscoelastic property and self-healing behavior. By crosslinking with 4% PEG-SG for only 3 min, the integrity of the cell-laden construct can last for 21 days due to the stable internal and external GEL/PEG-SG networks, and cells manifested long-term viability and spreading morphology. Nerve-like, muscle-like, and cartilage-like in vitro constructs exhibited homogeneous cell growth and remarkable biological specificities. This work provides not only a convenient and practical bioink for tissue engineering, targeted cell therapy, but also a new direction for hydrogel bioink development.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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