气相色谱外标法测定蛋黄卵磷脂（供注射用）中 7种脂肪酸含量Determination of 7 kinds of fatty acids in egg yolk lecithin(for injection) by GC with external standard method

Abstract

建立了适用于蛋黄卵磷脂（供注射用）中棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸、花生四烯酸、二十二碳六烯酸含量测定的分析方法。样品在具塞试管直接水浴加热甲酯化，正庚烷常温振荡提取后进气相色谱仪在起始柱温210 ℃下进行分析，利用外标法计算7种脂肪酸的含量。方法的专属性、精密度、准确度、耐用性考察均符合规定，在一定的质量浓度范围内，7种脂肪酸甲酯质量浓度与峰面积具有良好的线性关系，相关系数均大于0.999。该方法适用于蛋黄卵磷脂（供注射用）中7种脂肪酸含量的测定。 The method for the determination of palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, arachidonic acid and docosahexaenoic acid in egg yolk lecithin (for injection) was established.The samples were methylated by direct water bath heating in stoppered tubes,extracted by shaking with n-heptane at room temperature and analyzed by gas chromatography with initial column temperature 210 ℃,then the contents of 7 kinds of fatty acids were calculated by external standard method.The specificity, precision, accuracy and durability of the method were in line with the requirements, and in a range of mass concentrations,there was a linear relationship between the mass concentration of 7 kinds of fatty acid methyl esters and peak areas with all correlation coefficients greater than 0.999.So the method was suitable for the determination of 7 kinds of fatty acids in egg yolk lecithin (for injection).

Keywords

• 蛋黄卵磷脂（供注射用）；脂肪酸；气相色谱法；外标法
• egg yolk lecithin(for injection); fatty acid; gas chromatography; external standard method