Expression of periodontal ligament cell population miR ⁃ 155 in patients with chronic periodontitis

Abstract

Objective To understand the expression of miR⁃155 in the periodontal ligament cell population of pa⁃ tients with chronic periodontitis. Methods Periodontal ligament cells from the teeth of patients (n = 11) with chronic periodontitis were isolated and cultured, as were periodontal ligament cells from intact teeth obtained from healthy pa⁃ tients (n = 13) who had been treated with orthodontic extraction (the control group). The expression level of miR⁃155 in the two groups of periodontal ligament cell populations was detected by real⁃time fluorescence⁃based quantitative PCR. Results The periodontal ligament cell expression level of miR⁃155 was significantly higher in the chronic periodontitis group (0.93 ± 0.12) than in the control group (0.76 ± 0.25) (P < 0.01). Conclusion miR⁃155 expression in periodontal ligament cells was obviously stronger in patients with chronic periodontitis.

Keywords

• Chronic periodontitis
• Periodontal ligament cell population
• Micro RNA
• Micro⁃155
• Regulate