MdDREB2A in apple is involved in the regulation of multiple abiotic stress responses

Xinyu Lian; Xinyu Zhao; Qiang Zhao; Guiluan Wang; Yuanyuan Li; Yujin Hao

Horticultural Plant Journal (May 2021)

MdDREB2A in apple is involved in the regulation of multiple abiotic stress responses

Xinyu Lian,
Xinyu Zhao,
Qiang Zhao,
Guiluan Wang,
Yuanyuan Li,
Yujin Hao

Affiliations

Xinyu Lian: State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China
Xinyu Zhao: State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China
Qiang Zhao: College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong 266109, China
Guiluan Wang: State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China
Yuanyuan Li: State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China; Corresponding authors. Tel: +86 538 8246151
Yujin Hao: State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China; Corresponding authors. Tel: +86 538 8246151

Journal volume & issue: Vol. 7, no. 3
pp. 197 – 208

Abstract

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Abiotic stress has a serious effect on plant growth. The transcription factor DREB2A is a member of the AP2/ERF family, which is widely involved in abiotic stress response. However, the function of apple MdDREB2A has not been systematically investigated. In this study,MdDREB2A was isolated from the cultivar ‘Royal Gala’. The open reading frame of MdDREB2A was 1 197 bp in length and it encoded a protein of 398 amino acids with molecular weight of 43.8 kD. As a transcription factor, MdDREB2A was located in the nucleus. qRT-PCR analysis showed that MdDREB2A was involved in responses to drought, salt, and ABA stresses. Under these stress treatments, the relative electrical conductivity, superoxide anion and malondialdehyde (MDA) in transgenic materials significantly decreased, and the content of proline increased in MdDREB2A transgenic plants, compared to the controls, indicating that MdDREB2A transgenic apple calli and transgenic Arabidopsis exhibited improved resistance to abiotic stress. This study introduces a candidate gene for the genetic improvement of crop resistance and reveals important function of MdDREB2A in the regulation of abiotic stress response.

Published in Horticultural Plant Journal

ISSN: 2095-9885 (Print); 2468-0141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Plant culture
Website: https://www.keaipublishing.com/en/journals/horticultural-plant-journal/

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