Pengaruh Ekstrak Bayam Merah (Amaranthus gangeticus) terhadap Morfologi Stratum Hipokampus Model Anak Mencit Pascasapih Induk yang Terpapar Timbal Selama Masa Kehamilan

Abstract

Antioxidants in red spinach (Amaranthus gangeticus) extract are proposed to combat the toxicity of lead acetate in the central nervous system. The effect of red spinach extract to the morphology of post weaning mouse hippocampus model (Mus musculus) in pregnant mice that received oral lead acetate during pregnancy was analyzed. Twenty seven post-weaning mice offsprings were grouped into 3 groups: M0 (sodium carboxymethyl cellulose or CMC Na 0.5%), M1 (CMC Na 0.5%+lead acetate 1.16 mg/10 g of body weight/day) and M2 (CMC Na 0.5%+lead acetate 1.16 mg/10 g of body weight/day+red spinach extract 382.2 mg/10 g of body weight/day). Brains were histologically processed and stained with hematoxylin-eosin (HE). Width of stratum granulare (SG) of the dentate gyrus (GD) and each of stratum oriens-pyramidale (SOP) of CA1, CA2, CA3 from each mouse hippocampus were obtained and analyzed statistically using analysis of variance (ANOVA) and least significant difference (LSD) (p0.05). In conclusion, red spinach extract might dampen the adverse effects of oral lead acetate in post-weaning mouse hippocampus model from pregnant mice orally exposed to lead acetate during pregnancy.

Keywords

• Hippocampus
• lead acetate
• red spinach