Biosynthesis and characterisation of solid lipid nanoparticles and investigation of toxicity against breast cancer cell line

Abstract

Abstract Solid lipid nanoparticles (SLNs) comprise non‐toxic surface‐active lipidic agents combined with appropriate ratios of drugs or essential oils. The goal of this research was to investigate the effects of the SLN synthesised using essential oils of Foeniculum vulgare on the MCF‐7 breast cancer cell line. SLNs were prepared by homogenisation and ultrasound techniques and characterised by dynamic light scattering (DLS), zeta potential assessment, and transmission electron microscopy (TEM). 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay (MTT assay), flow‐cytometry, and Acridine‐Orange assay were employed for assessing the biological activities of the SLNs. The average particle size was 55.43 nm and the net surface charge was −29.54 ± 11.67 mV. TEM showed that the mean particle size was 33.55 nm and the synthesised SLNs had a uniform round morphology. The MTT assay showed that the prepared SLNs had high toxicity against MCF‐7 cells and low toxicity against normal HUVECs cells. Flow‐cytometry revealed a noteworthy rise in the subG1 peak of the cell cycle in the cancer cells treated with SLNs compared to the controls, indicating apoptosis in cancer cells. The results also showed discolouration in SLNs‐treated cells, which further confirmed the induction of apoptosis and the toxicity of the SLNs against MCF‐7 cells.