Quantitative Analysis of Phytocompounds and Gas Chromatography Mass Spectrometry Profiling of Methanolic Extract of Citrus Limon Peel: An Experimental Study

Abstract

Introduction: All citrus plants are rich sources of phytochemicals that have many beneficial effects on human health. Different parts of the Citrus Limon plant, such as fruits, leaves, seeds, stems, and peels, also contain a wide spectrum of phytochemicals with therapeutic effects. Aim: The present study focused on phytochemical analysis and Gas Chromatography Mass Spectrometry (GCMS) profiling of limon peel. Materials and Methods: An experimental qualitative analysis was conducted at Systemic Life Sciences and Research in Hyderabad, Telangana, India from April 2023 to April 2024. Fresh Citrus Limon fruits were collected from an authorised vendor, identified, and authenticated. The peels were dried, coarse powdered, and a methanolic extract was prepared using the Soxhlet extraction method. An experimental qualitative analysis GCMS was performed to identify the chemical compounds present in the Citrus Limon peel extract. Results: Phytochemical analysis of Citrus Limon peel showed the presence of various metabolites such as carbohydrates, proteins, alkaloids, tannins, flavonoids, saponins, steroids, terpenoids, and glycosides. The GCMS profiling identified 13 chemical compounds, most notably Pentamethoxyflavone, Tetramethoxyflavone, and 2-Methoxy-4-vinylphenol in the methanolic extract of Citrus Limon peel. Conclusion: It was concluded that the bioactive phytocomponents are abundant in Citrus Limon peel extract.

Keywords

• alkaloids
• flavonoids
• phytocomponents
• polymethoxyflavone