A perioperative layered autologous tissue expansion graft for hollow organ repair

Oliver Willacy; Nikolai Juul; Loai Taouzlak; Clara I. Chamorro; Fatemeh Ajallouiean; Magdalena Fossum

Heliyon (Feb 2024)

A perioperative layered autologous tissue expansion graft for hollow organ repair

Oliver Willacy,
Nikolai Juul,
Loai Taouzlak,
Clara I. Chamorro,
Fatemeh Ajallouiean,
Magdalena Fossum

Affiliations

Oliver Willacy: Laboratory of Tissue Engineering, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Division of Pediatric Surgery, Department of Surgery and Transplantation, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
Nikolai Juul: Laboratory of Tissue Engineering, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Division of Pediatric Surgery, Department of Surgery and Transplantation, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
Loai Taouzlak: Department of Health Technology, Technical University of Denmark, 2800: Kgs, Lyngby, Denmark
Clara I. Chamorro: Laboratory of Tissue Engineering, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden
Fatemeh Ajallouiean: Department of Health Technology, Technical University of Denmark, 2800: Kgs, Lyngby, Denmark; The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Ørsted Plads, 2800: Kgs, Lyngby, Denmark
Magdalena Fossum: Laboratory of Tissue Engineering, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Division of Pediatric Surgery, Department of Surgery and Transplantation, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark; Laboratory of Tissue Engineering, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden; Corresponding author: M. Fossum, Laboratory of Tissue Engineering, Department of Women's and Chidren's Health, Biomedicum floor 4, Karolinska Institutet, 117 77, Stockholm, Sweden.

Journal volume & issue: Vol. 10, no. 3
p. e25275

Abstract

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Tissue engineering has not been widely adopted in clinical settings for several reasons, including technical challenges, high costs, and regulatory complexity. Here, we introduce the Perioperative Layered Autologous Tissue Expansion graft (PLATE graft), a composite biomaterial and collagen-reinforced construct with autologous epithelium on one side and smooth muscle tissue on the other. Designed to mimic the structure and function of natural hollow organs, the PLATE graft is unique in that it can be produced in a standard operating theatre and is cost-effective. In this proof-of-principle study, we test its regenerative performance in eight different organs, present biomechanical and permeability tests, and finally explore its in vivo performance in live rabbits.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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