Journal of Contemporary Medicine (May 2022)
DOES THE DISINFECTANT EFFICACY OF THE 2% GLUTERALDEHIDE SOLUTION CHANGE WITH THE AGING OF THE SOLUTION?
Abstract
Purpose:In our study, it was aimed to determine whether the disinfectant efficacy of 2% glutaraldehyde solution changes with the aging of the solution. Methods: In our study, metal obtained from k-wire, plastic obtained from serum sets, and glass balls were used to imitate metal, plastic, and glass materials found in surgical instruments. 20 of each material were contaminated with Klebsiella pneumoniae (K. pneumoniae), Staphylococcus aureus (S. aureus),Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli), and Yeast on the 1st, 14th, and 28th days of the solution. 10 of them were thrown into 2% glutaraldehyde solution in different glasses and kept in the solution for 15 minutes. Subsequently, materials were taken from the glasses using separate sterile forceps for each piece. The other 10 were washed with physiological saline solution for 1 minute. After the procedures, they were sent to the microbiology laboratory without waiting and inoculated on routine media, and Gram staining was performed simultaneously. The identification of strains was carried out by conventional methods. Results: No growth was detected on the 1st, 14th, and 28th days in all metal, plastic, and glass materials disinfected with 2% alkaline glutaraldehyde solution. In the control groups, growth rates were 53.3%, 50%, and 63.3%, respectively. Conclusions: If the guidelines and manufacturer's recommendations are followed in the preparation and use of 2% alkaline glutaraldehyde solution, it provides an effective disinfection without being affected by the aging of the solution.
