IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium

Jenna L Wingfield; Ilaria Mengoni; Heather Bomberger; Yu-Yang Jiang; Jonathon D Walsh; Jason M Brown; Tyler Picariello; Deborah A Cochran; Bing Zhu; Junmin Pan; Jonathan Eggenschwiler; Jacek Gaertig; George B Witman; Peter Kner; Karl Lechtreck

doi:10.7554/eLife.26609

eLife (May 2017)

IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium

Jenna L Wingfield,
Ilaria Mengoni,
Heather Bomberger,
Yu-Yang Jiang,
Jonathon D Walsh,
Jason M Brown,
Tyler Picariello,
Deborah A Cochran,
Bing Zhu,
Junmin Pan,
Jonathan Eggenschwiler,
Jacek Gaertig,
George B Witman,
Peter Kner,
Karl Lechtreck

Affiliations

Jenna L Wingfield: Department of Cellular Biology, University of Georgia, Athens, United States
Ilaria Mengoni: Department of Cellular Biology, University of Georgia, Athens, United States
Heather Bomberger: Department of Cellular Biology, University of Georgia, Athens, United States; College of Engineering, University of Georgia, Athens, United States
Yu-Yang Jiang: Department of Cellular Biology, University of Georgia, Athens, United States
Jonathon D Walsh: Department of Genetics, University of Georgia, Athens, United States
Jason M Brown: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States; Department of Biology, Salem State University, Salem, United States
Tyler Picariello: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States
Deborah A Cochran: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States
Bing Zhu: MOE Key Laboratory of Protein Sciences, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
Junmin Pan: MOE Key Laboratory of Protein Sciences, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Jonathan Eggenschwiler: Department of Genetics, University of Georgia, Athens, United States
Jacek Gaertig: Department of Cellular Biology, University of Georgia, Athens, United States
George B Witman: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States
Peter Kner: College of Engineering, University of Georgia, Athens, United States
Karl Lechtreck: ORCiD; Department of Cellular Biology, University of Georgia, Athens, United States

DOI: https://doi.org/10.7554/eLife.26609
Journal volume & issue: Vol. 6

Abstract

Read online

Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic proteins in cilia. To study how trains assemble, we employed fluorescence protein-tagged IFT proteins in Chlamydomonas reinhardtii. IFT-A and motor proteins are recruited from the cell body to the basal body pool, assembled into trains, move through the cilium, and disperse back into the cell body. In contrast to this ‘open’ system, IFT-B proteins from retrograde trains reenter the pool and a portion is reused directly in anterograde trains indicating a ‘semi-open’ system. Similar IFT systems were also observed in Tetrahymena thermophila and IMCD3 cells. FRAP analysis indicated that IFT proteins and motors of a given train are sequentially recruited to the basal bodies. IFT dynein and tubulin cargoes are loaded briefly before the trains depart. We conclude that the pool contains IFT trains in multiple stages of assembly queuing for successive release into the cilium upon completion.

flagella
IFT

Published in eLife

ISSN: 2050-084X (Online)
Publisher: eLife Sciences Publications Ltd
Country of publisher: United Kingdom
LCC subjects: Medicine; Science: Biology (General)
Website: https://elifesciences.org

About the journal

Abstract

Keywords