Anti-HIV-1 integrase activity of compounds from Cassia garrettiana heartwood

Abstract

The ethanol extract of heartwood from Cassia garrettiana had strong anti-HIV-1 integrase (IN) activity with an IC50 value of 3.0 µg/mL. Therefore, its fractions and compounds were investigated for their anti-HIV-1 IN effect using the multiplate integration assay (MIA). From bioassay-guided isolation, the ethyl acetate fraction was then separated to give five compounds which are chrysophanol (1), piceatannol (2), aloe-emodin (3), emodin (4) and cassigarol E (5). Of the tested samples, piceatannol (2) showed the highest activity against HIV-1 IN with an IC50 of 17.9 µM, followed by cassigarol E (5, IC50 = 72.9 µM), respectively. From the present study, this is the first report on anti-HIV-1 IN activity of Cassia garrettiana.

Keywords

• HIV-1 integrase
• Cassia garrettiana
• Caesalpiniaceae