Nature Communications (Apr 2022)

Direct measurements of mRNA translation kinetics in living cells

  • Mikhail Metelev,
  • Erik Lundin,
  • Ivan L. Volkov,
  • Arvid H. Gynnå,
  • Johan Elf,
  • Magnus Johansson

DOI
https://doi.org/10.1038/s41467-022-29515-x
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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Metelev et al. use single-molecule tracking to study kinetics of translation directly in E. coli cells, and how it is affected by translation inhibitors and rRNA mutations. Their results support widespread 70S re-initiation on mRNAs.